ABSTRACT.- The intensification of irrigated rice production systems requires a comprehensive assessment of the environmental impact of pesticide use. Here, we investigated the behavior of the herbicide glyphosate (GLY) and its metabolite aminophosphonic acid (AMPA), throughout four rice rotations (continuous rice cropping (RC), ricesoybean (R-S), rice-short term pasture (R-PS) and rice-long term pasture R-PL) reflecting different agricultural intensification scenarios in a long-term experimental (LTE) site. © 2025 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.