ABSTRACT.- The antifungal activity of Chenopodium ambrosioides essential oil against Penicillium digitatum was evaluated in vitro (liquid phase) and in vivo on artificially inoculated Okitsu Satsuma mandarin (Citrus unshiu). In vitro, the essential oil exhibited a fungicidal effect, with 100% effectiveness at a minimum inhibitory concentration of 29 uL/mL, and a minimum fungicidal concentration of 125 uL/mL. However, in vivo, P. digitatum grew on over 80% of treated fruit, showing no significant

ABSTRACT.- A simple and fast analytical method was developed and applied to assess the effect of two forms of zinc fertilization on a pecan tree cultivar in Uruguay: fertigation and foliar application with a specially formulated fertilizer. Zinc content was determined in 36 leaf samples from two crop cycles: 2020-2021 and 2021-2022. Fresh samples were dried, ground, and sieved. Analytical determinations were performed by flame atomic absorption spectrometry (FAAS, considered a standard method) and energy dispersive X-ray spectrometry (EDXRF, the proposed method). © 2025 by the authors.

ABSTRACT. - Zygote microinjection is considered the most suitable technique to introduce CRISPR/Cas9 reagents for efficient genome editing in livestock. In this study, zygote electroporation was evaluated as an alternative to microinjection for CRISPR/Cas9-mediated genome editing in sheep. Four experiments were conducted on 3548 cumulusoocyte complexes. Acid Tyrode's solution (AT) was used to partially degrade the zona pellucida (ZP) to improve reagent entry, resulting in ZP thinning with longer AT exposure (P < 0.05).

While agriculture is crucial to feeding people, it is strongly impacted by climate change and variability, and also contributes to it. Agriculture and land use change are responsible for around ¼ of greenhouse gas emissions from human activities(1). Climate change already impacts the quantity, quality and geographic distribution of food production from agriculture. In this special issue, entitled "Climate change and variability impacts on agriculture", the selected articles address different issues.

ABSTRACT.- Uruguay's livestock methane is a mayor source of greenhouse gas (GHG) emissions, despite the country's low overall emissions. A case study was conducted on Forestal Caja Bancaria (FCB), a commercial farm in Uruguay's central region, with an integrated production system covering 5,802 hectares. The study aimed to estimate GHG emissions from livestock, crops and forestry, CO2 captured by eucalypt and pine plantations, and soil organic carbon for all land uses. -------------------------

ABSTRACT.- For years, livestock production has been accused of having a supposed impact on global warming. This message permeated broad sectors of public opinion. Recently, questions have arisen about the metrics used to determine the potential contribution of different greenhouse gases.

ABSTRACT.- The agricultural sector must mitigate losses in greenhouse gas (GHG) emissions, nutrients, and inputs within the context of climate change and ecosystem degradation. Integrated crop-livestock production systems can enhance carbon and nutrient circularity. A holistic methodology is proposed to guide producers in developing strategies that reduce environmental impacts while improving system resilience through circular and ecosystem based practices. Developed as part of the Integrity project (EraNet), this methodology presents a graduated approach organized into four stages.

The present study aimed to determine the content of the bioactive compounds: anserine, carnosine, L-carnitine, coenzyme Q10, glutathione, and taurine in raw and cooked beef aged for 5 or 90 days.

ABSTRACT.- Leaf rust (LR) of bread wheat (Triticum aestivumL.), caused by the fungus Puccinia triticina Eriks, is one of the most important diseases in Paraguay, the Southern Cone of America and worldwide. The economic importance of the disease is clear considering that two or more fungicide applications are necessary to control the disease in susceptible cultivars. The best strategy for the management of this disease is through genetic resistance.