Rice blast, caused by Pyricularia oryzae, is one of the most destructive diseases of rice in the world. In Uruguay, approximately 35% of the rice area was cultivated with susceptible cultivars in 2022-2023. The blast fungus shows a high degree of variability in the field, and limited information is available on its genetic diversity and population structure in Uruguay. Knowing the genetic variability of this pathogen will provide information useful for genetic improvement programs.