ABSTRACT. The methodology of a spaced plant seed increase nursery to produce pre-basic seed of the subtropical forage legume Lotononis bainesii Baker cv. "INIA Glencoe" is presented. Twenty grams of nucleus seed were grown for 2 months in a glasshouse in a forestry nursery. The best 30 000 plants were transplanted in September 2002 in a 1 × 1 m grid to a seed increase area at Glencoe research station of INIA Tacuarembó, Uruguay.

ABSTRACT. Stress conditions are known to promote reproductive sprouting and flowering in Citrus. However, signalling involved in stress perception and flowering induction remains obscure. To gain insight into the mechanism involved in stress signalling, the aim of this work was to study the physiological and biochemical changes triggered by water stress and their relationship with budbreak in ?Valencia? orange (C. sinensis (L.) Osb.) and ?Ellendale? tangor (C. reticulata Bl.). Water stress was promoted by subjecting plants to 70-days of water deficit (water potential:Ψw

ABSTRACT. We evaluated the effects of the soil temperature (15, 25 and 35°C) and soil anoxia from flooding for 20 days on citrus rootstock seedlings of CT33 trifoliate, Carrizo citrange and Troyer citrange along with Tucuman, Pomeroy and Rubidoux trifoliates in a greenhouse. Flooding had no effect on shoot water potential (Ψs) at 25°C but at 15°C and 35°C, there were differences from non-flooded trees. Carrizo and Troyer had higher Ψs than CT33, and Tucuman had the highest Ψs among the trifoliates. Soil temperature was more important than flooding in determining plant water relations.

ABSTRACT. We tested the effect of soil hypoxia from flooding on fruit drop in a mature orchard of ?Satsuma mandarin? (Citrus unshiu Marcow) trees on Pomeroy (Poncirus trifoliate (L.) Raf.) rootstock. Trees were flooded for 30 days with 10 cm water above the soil surface using a 60 cm high aluminum barrier which was buried to depth a 45 cm around the trees. Flooding began at flower anthesis and lasted until early fruit set. Flooding reduced the soil oxygen levels from 5 to 2 µg O2 cm-2 min-1.

ABSTRACT. Standard molecular methods for plant virus diagnosis require the purification of RNA or DNA extracts from a large number of samples, with sufficient concentration and quality for their use in PCR, RT-PCR, or qPCR analysis. Most methods are laborious and use either hazardous and/or costly chemicals.Apreviously published protocol for RNA isolation from several plant species yields high amounts of good quality RNADNA mixture in a simple, safe and inexpensive manner.

ABSTRACT. The effect of shearing ewes at 50 days of gestation on birth and weaning weight, vigour of the lambs in the first hour of life and the survival of single and twin lambs was investigated.

Abstract:The Kenyan wheat (Triticum aestivum L.) ?Kenya Kongoni ?exhibits high levels of adult plant resistance (APR) to leaf rust (LR) and yellow rust (YR). We determined the genomic regions associated with LR and YR resistance in a population of 148 recombinant inbred lines generated from a cross between ? Avocet-YrA ?and Kenya Kongoni. Field experi-ments to characterize APR to LR and YR were conducted in four and two Mexican or Uruguayan environments, respectively.