The aim of this study was to evaluate the effects of pre slaughter lairage time and temperament on glycogen content. Fourteen Hereford steers fed on pasture were assigned to two treatments (T) according to lairage time:3 hours (T1, n = 7) and 12 hours (T2, n = 7). Individual temperament was assessed using crush score and flight speed. Muscle samples were extracted from Longissimus thoracis et lumborum (LM) muscles 45 minutes post mortem, to determine glycogen content. To evaluate the effect of lairage time and temperament on glycogen content, analysis of variance was used fitting a general linear model including the fixed effects of lairage time, CS, and FS in classes. Adjusted means were compared by the Tukey test and the results were considered statistically significant when P