RESUMEN La micropropagación mediante cultivo in vitro de plantas permite la producción a gran escala de individuos genéticamente idénticos al material de partida. Las especies leñosas tienen dificultades en la etapa de aclimatación por la lentitud que presentan en el desarrollo de respuestas fisiológicas y morfológicas a los cambios de ambiente. El éxito final de la propagación in vitro depende de la capacidad de transferencia de las plantas desde el laboratorio a las condiciones de invernáculo.

ABSTRACT. The anther culture response in Solanum commersonii (2n = 2x = 24, 1EBN) and S. chacoense (2n = 2x = 24, 2EBN), two wild potato germplasm resources was studied to obtain haploid plants. Three accessions from each of the two species and 3200 anthers from each genotype were cultured. Authors assessed different culture media; ascorbic acid, L?cysteine and silver nitrate (AgNO3) were included to prevent browning of anther cultures. Addition of AgNO3, was effective to induce embryogenesis. The clones from S. commersonii showed different embryogenic response to androgenesis.

ABSTRACT. Aspergillus spp. and Penicillium spp. are the main producers of ochratoxin A (OTA), a mycotoxin responsible for fatal human diseases. Some authorities have established a maximum of 2 μg/L of OTA in wine. Although the incidence and occurrence of OTA in grapes and wine is highly related to climate conditions, as has been extensively documented, there is no conclusive information on the effects of cultivation systems on the presence of OTA.

ABSTRACT. Five potatoes varieties both raw and microwave (800 W, 8 min) and fried (180°C, 5 min) were evaluated. Colored-fleshed potatoes showed between 2- and 2.5-fold higher total polyphenols (TP, 2880.5?3241.6 mg GAE/kg DW) than the light-yellow. Chlorogenic acid was predominant. However, related to the others identified phenols, microwave and fried presented different phenolic profile. Vitamin C and total antioxidant capacity were also higher on colored ones with values in the range of 260.7?511.6 mg AA/kg DW and 344.1?527.8 mg GAE/kg DW respectively.

ABSTRACT. The aim of the experiment was to evaluate the reproductive performance of ewes synchronized with two doses of prostaglandin F2a (PG) at different intervals and inseminated at a fixed time. During the breeding season (April to June), 370 multiparous Corriedale ewes were assigned to five groups according to body condition score and body weight, and synchronized with two doses of PG administered 7, 10, 12, 14 or 16 days apart (groups PG7, PG10, PG12, PG14 or PG16; n ¼ 73, 76, 74, 72, 75; respectively).