ABSTRACT. The methodology of a spaced plant seed increase nursery to produce pre-basic seed of the subtropical forage legume Lotononis bainesii Baker cv. "INIA Glencoe" is presented. Twenty grams of nucleus seed were grown for 2 months in a glasshouse in a forestry nursery. The best 30 000 plants were transplanted in September 2002 in a 1 × 1 m grid to a seed increase area at Glencoe research station of INIA Tacuarembó, Uruguay.